Use random-access in vivo with Ultrafast Local Volume Excitation (ULoVE)

Sample movement in vivo can compromise the stability of random access recordings. To suppress these movement artefacts we expand the focal volume in 3D through an optimal combination of holographic patterning and fast scanning, to create ultrafast local volume excitation (ULoVE). Our Acousto-optic light steering technology combines the best of three worlds: Random Access, Fast local 3D scanning and holographic patterning.

Ultrafast Two-Photon Imaging of a High-Gain Voltage Indicator in Awake Behaving Mice
Vincent Villette, Mariya Chavarha, Ivan K. Dimov, ..., Jun Ding, Stephane Dieudonne, Michael Z. Lin, Cell 2019

Fast two-photon volumetric imaging of an improved voltage indicator reveals electrical activity in deeply located neurons in the awake brain
Mariya Chavarha et al, Biorxiv 2018